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1200 series hplc system  (Agilent technologies)


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    Structured Review

    Agilent technologies 1200 series hplc system
    1200 Series Hplc System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 98/100, based on 2085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hplc+1200+series/PLRP-S/pm39838819__bc4c00457_si_001-14-13-12
    Average 98 stars, based on 2085 article reviews
    1200 series hplc system - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Ammonium based ionic liquids useful for lignocellulosic processing
    Article Snippet: .. The filtrate was neutralized with CaCO3 and monomeric sugars were determined from the filtrate by Agilent HPLC 1200 Series equipped with a Bio-Rad Aminex HPX-87P column and a Refractive Index detector (aqueous mobile phase, 0.6 mL/min, column temperature 85° C.). ..

    Article Title: Does the Addition of Low-Dose Antibiotics Compromise the Mechanical Properties of Polymethylmethacrylate (PMMA)?
    Article Snippet: .. Concerning the LC-MS/MS conditions, chromatographic separation was performed on a modular HPLC 1200 Series (Agilent Technologies, Waldbronn, Germany) using a Luna C18 (II) column, 150 × 2 mm, with two C18, 4 × 2 mm, guard columns (Phenomenex, Aschaffenburg, Germany) thermostated at 25 °C. ..

    Article Title: Ketoreductase polypeptides for the preparation of phenylephrine
    Article Snippet: TABLE 9 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Mightysil Aqua RP18, 250 × 4.6 mm, 5 μm Mobile Phase 98.5% (0.25% NaOAc; pH 5.5)/1.5% MeCN Flow Rate 1.0 mL/min Column Temperature 40° C. Detection Wavelength 275 nm Injection Volume 10 μL Run Time 10 min Retention Time Substrate: 6.1 min; Phenylephrine: 7.5 min. .. TABLE 9 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Mightysil Aqua RP18, 250 × 4.6 mm, 5 μm Mobile Phase 98.5% (0.25% NaOAc; pH 5.5)/1.5% MeCN Flow Rate 1.0 mL/min Column Temperature 40° C. Detection Wavelength 275 nm Injection Volume 10 μL Run Time 10 min Retention Time Substrate: 6.1 min; Phenylephrine: 7.5 min. ..

    Article Title: Ketoreductase polypeptides for the preparation of phenylephrine
    Article Snippet: TABLE 10 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Regis CBH 4.0 × 100 mm (5 μm) Mobile Phase 10% MeOH + 90% (8 mM NH4OAc + 13 μM EDTA; pH 5.5) Flow Rate 0.8 mL/min Column Temperature Ambient Detection Wavelength 275 nm Injection Volume 10 μL Run Time 5 min Retention Time R (L): 2.6 min; S(D): 3.2 min Item Analytical Parameter LOD 0.11 mg/L, S/N > 3 LOQ 0.32 mg/L, S/N > 10 A typical chromatogram from this method showed a peak at about 2.6 min and a slightly shorter broader peak at about 3.3 min. .. TABLE 10 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Regis CBH 4.0 × 100 mm (5 μm) Mobile Phase 10% MeOH + 90% (8 mM NH4OAc + 13 μM EDTA; pH 5.5) Flow Rate 0.8 mL/min Column Temperature Ambient Detection Wavelength 275 nm Injection Volume 10 μL Run Time 5 min Retention Time R (L): 2.6 min; S(D): 3.2 min Item Analytical Parameter LOD 0.11 mg/L, S/N > 3 LOQ 0.32 mg/L, S/N > 10 A typical chromatogram from this method showed a peak at about 2.6 min and a slightly shorter broader peak at about 3.3 min. ..

    Article Title: In Situ Eutectic Formation in a Polymeric Matrix via Hot-Melt Reactive Extrusion and the Use of Partial Least Squares Regression Modeling for Reaction Yield Determination
    Article Snippet: .. The analysis was carried out using an Agilent HPLC 1200 series, consisting of a binary pump, degasser, column oven, and variable wavelength UV detector, from Agilent Technologies, UK. .. Samples were analyzed using a Zorbax C 18 column (100 mm × 4.6 mm with 3.5 μm packing) from Agilent Technologies, UK, at 228 nm.

    Article Title: Ketoreductase polypeptides for the preparation of phenylephrine
    Article Snippet: TABLE 7 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Mightysil Aqua RP18, 250 × 4.6 mm, 5 μm Mobile Phase 93% (0.25% NaOAc; pH 5.0)/7% MeCN Flow Rate 1.20 mL/min Column Temperature 40° C. Wavelength 275 nm Injection Volume 10 μL Run time: 6 min Retention time Product (3.3 min); Substrate (3.8 min) Item Analytical Parameter Linearity R = 1.0 (substrate); Linear Range = 0-817 mg/L R = 0.99995 (Product); Linear Range = 69.5-400 mg/L LOD 0.51 mg/L (Product); 0.17 mg/L (Substrate) LOQ 1.54 mg/L (Product); 0.43 mg/L (Substrate) An exemplary chromatogram obtained by this method under isocratic conditions showed a phenylephrine peak at 3.224 min and a slightly broader substrate peak at 3.701 min which is about two-thirds height of the phenylephrine peak. .. TABLE 7 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Mightysil Aqua RP18, 250 × 4.6 mm, 5 μm Mobile Phase 93% (0.25% NaOAc; pH 5.0)/7% MeCN Flow Rate 1.20 mL/min Column Temperature 40° C. Wavelength 275 nm Injection Volume 10 μL Run time: 6 min Retention time Product (3.3 min); Substrate (3.8 min) Item Analytical Parameter Linearity R = 1.0 (substrate); Linear Range = 0-817 mg/L R = 0.99995 (Product); Linear Range = 69.5-400 mg/L LOD 0.51 mg/L (Product); 0.17 mg/L (Substrate) LOQ 1.54 mg/L (Product); 0.43 mg/L (Substrate) An exemplary chromatogram obtained by this method under isocratic conditions showed a phenylephrine peak at 3.224 min and a slightly broader substrate peak at 3.701 min which is about two-thirds height of the phenylephrine peak. ..

    Article Title: Evaluation of Gentamicin Release of PMMA Cements Using Different Methods: HPLC, Elution and Inhibition Zone Testing
    Article Snippet: .. LC-MS/MS chromatographic separation was performed on a modular HPLC 1200 Series (Agilent Technologies, Waldbronn, Germany) using a Luna C18 (II) column, 150 × 2 mm, with two C18 4 × 2 mm guard columns (Phenomenex, Aschaffenburg, Germany) at 25 °C. ..

    Refractive Index:

    Article Title: Ammonium based ionic liquids useful for lignocellulosic processing
    Article Snippet: .. The filtrate was neutralized with CaCO3 and monomeric sugars were determined from the filtrate by Agilent HPLC 1200 Series equipped with a Bio-Rad Aminex HPX-87P column and a Refractive Index detector (aqueous mobile phase, 0.6 mL/min, column temperature 85° C.). ..

    Injection:

    Article Title: Ketoreductase polypeptides for the preparation of phenylephrine
    Article Snippet: TABLE 9 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Mightysil Aqua RP18, 250 × 4.6 mm, 5 μm Mobile Phase 98.5% (0.25% NaOAc; pH 5.5)/1.5% MeCN Flow Rate 1.0 mL/min Column Temperature 40° C. Detection Wavelength 275 nm Injection Volume 10 μL Run Time 10 min Retention Time Substrate: 6.1 min; Phenylephrine: 7.5 min. .. TABLE 9 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Mightysil Aqua RP18, 250 × 4.6 mm, 5 μm Mobile Phase 98.5% (0.25% NaOAc; pH 5.5)/1.5% MeCN Flow Rate 1.0 mL/min Column Temperature 40° C. Detection Wavelength 275 nm Injection Volume 10 μL Run Time 10 min Retention Time Substrate: 6.1 min; Phenylephrine: 7.5 min. ..

    Article Title: Ketoreductase polypeptides for the preparation of phenylephrine
    Article Snippet: TABLE 10 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Regis CBH 4.0 × 100 mm (5 μm) Mobile Phase 10% MeOH + 90% (8 mM NH4OAc + 13 μM EDTA; pH 5.5) Flow Rate 0.8 mL/min Column Temperature Ambient Detection Wavelength 275 nm Injection Volume 10 μL Run Time 5 min Retention Time R (L): 2.6 min; S(D): 3.2 min Item Analytical Parameter LOD 0.11 mg/L, S/N > 3 LOQ 0.32 mg/L, S/N > 10 A typical chromatogram from this method showed a peak at about 2.6 min and a slightly shorter broader peak at about 3.3 min. .. TABLE 10 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Regis CBH 4.0 × 100 mm (5 μm) Mobile Phase 10% MeOH + 90% (8 mM NH4OAc + 13 μM EDTA; pH 5.5) Flow Rate 0.8 mL/min Column Temperature Ambient Detection Wavelength 275 nm Injection Volume 10 μL Run Time 5 min Retention Time R (L): 2.6 min; S(D): 3.2 min Item Analytical Parameter LOD 0.11 mg/L, S/N > 3 LOQ 0.32 mg/L, S/N > 10 A typical chromatogram from this method showed a peak at about 2.6 min and a slightly shorter broader peak at about 3.3 min. ..

    Article Title: Ketoreductase polypeptides for the preparation of phenylephrine
    Article Snippet: TABLE 7 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Mightysil Aqua RP18, 250 × 4.6 mm, 5 μm Mobile Phase 93% (0.25% NaOAc; pH 5.0)/7% MeCN Flow Rate 1.20 mL/min Column Temperature 40° C. Wavelength 275 nm Injection Volume 10 μL Run time: 6 min Retention time Product (3.3 min); Substrate (3.8 min) Item Analytical Parameter Linearity R = 1.0 (substrate); Linear Range = 0-817 mg/L R = 0.99995 (Product); Linear Range = 69.5-400 mg/L LOD 0.51 mg/L (Product); 0.17 mg/L (Substrate) LOQ 1.54 mg/L (Product); 0.43 mg/L (Substrate) An exemplary chromatogram obtained by this method under isocratic conditions showed a phenylephrine peak at 3.224 min and a slightly broader substrate peak at 3.701 min which is about two-thirds height of the phenylephrine peak. .. TABLE 7 Chromatographic Conditions Instrument Agilent HPLC 1200 series Column Mightysil Aqua RP18, 250 × 4.6 mm, 5 μm Mobile Phase 93% (0.25% NaOAc; pH 5.0)/7% MeCN Flow Rate 1.20 mL/min Column Temperature 40° C. Wavelength 275 nm Injection Volume 10 μL Run time: 6 min Retention time Product (3.3 min); Substrate (3.8 min) Item Analytical Parameter Linearity R = 1.0 (substrate); Linear Range = 0-817 mg/L R = 0.99995 (Product); Linear Range = 69.5-400 mg/L LOD 0.51 mg/L (Product); 0.17 mg/L (Substrate) LOQ 1.54 mg/L (Product); 0.43 mg/L (Substrate) An exemplary chromatogram obtained by this method under isocratic conditions showed a phenylephrine peak at 3.224 min and a slightly broader substrate peak at 3.701 min which is about two-thirds height of the phenylephrine peak. ..

    other:

    Article Title: Lubricant-Coated Organ-on-a-Chip for Enhanced Precision in Preclinical Drug Testing.
    Article Snippet: HPLC: The medium samples were mixed with acetonitrile and LCMS grade, in a 1:1 ratio and then centrifuged at 4 °C at 15 000 rpm for 10 min. After centrifugation, A 50-μL aliquot of the supernatant was collected and subjected to HPLC (HPLC 1200 Series, Agilent Technologies) for the quantification of dopamine and levodopa.



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